Hotspot

rps18-trnG-UCC

Genome · 31599-31725 · 127 bp

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Pi 0.218

MarkerSeek score 74.10

Primer available yes

Label rps18-trnG-UCC

Feature Metadata

label_name: rps18-trnG-UCC
feature_type: igs
region: Genome
length_bp: 127
variable_sites: 46
pi: 0.218
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-trnG-UCC_p1	GCCTTCCCAAAATGAAAGGC	TTCAATAAAAGTCCCCCGCC	50.0	50.0	57.9	58.2	1101 bp	0.2	45.9
rps18-trnG-UCC_p2	TCCTTTCATTTTGGGGGCAA	TTCAATAAAAGTCCCCCGCC	45.0	50.0	57.9	58.2	1058 bp	0.2	45.9
rps18-trnG-UCC_p3	TCAATCGAGTCACCCGTCTA	TTCAATAAAAGTCCCCCGCC	50.0	50.0	57.9	58.2	908 bp	0.2	45.8
rps18-trnG-UCC_p4	ACTATATCCGCAAAAGGCCG	TTCAATAAAAGTCCCCCGCC	50.0	50.0	58.1	58.2	1136 bp	0.2	45.8
rps18-trnG-UCC_p5	GCCTTCCCAAAATGAAAGGC	AACGAAAAATGGGAGGGAGG	50.0	50.0	57.9	57.8	1065 bp	0.2	45.5