Hotspot

rpoB-trnC-GCA

Genome · 26329-27461 · 1133 bp

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Pi 0.0268

MarkerSeek score 74.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: Genome
length_bp: 1133
variable_sites: 57
pi: 0.0268
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GCCCAGGTATTACAGCCATT	AAAGGGGATTTCGCGTCTTT	50.0	45.0	57.6	58.1	1274-1340 bp	1.0	83.3
rpoB-trnC-GCA_p2	GCCCAGGTATTACAGCCATT	AAGGGGATTTCGCGTCTTTT	50.0	45.0	57.6	58.1	1273-1339 bp	1.0	83.3
rpoB-trnC-GCA_p3	GCCCTGATCAACGAACCTAC	AAAAGGGGATTTCGCGTCTT	55.0	45.0	58.3	58.1	1322-1388 bp	1.0	83.2
rpoB-trnC-GCA_p4	GCCCTGATCAACGAACCTAC	AAAGGGGATTTCGCGTCTTT	55.0	45.0	58.3	58.1	1321-1387 bp	1.0	83.2
rpoB-trnC-GCA_p5	GCCCTGATCAACGAACCTAC	AAGGGGATTTCGCGTCTTTT	55.0	45.0	58.3	58.1	1320-1386 bp	1.0	83.2