Hotspot

ndhK

LSC · 51154-51837 · 684 bp

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Pi 0.00731

MarkerSeek score 63.00

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 684
variable_sites: 5
pi: 0.00731
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TCCCCCTTTCTTATTCTGTACA	GGTTCAGTTTATGCTTGGCG	40.9	50.0	56.2	57.7	820-824 bp	1.0	47.7
ndhK_p2	TCCCCCTTTCTTATTCTGTACAA	GGTTCAGTTTATGCTTGGCG	39.1	50.0	56.9	57.7	820-824 bp	1.0	45.5
ndhK_p3	TTCCCCCTTTCTTATTCTGTACA	GGTTCAGTTTATGCTTGGCG	39.1	50.0	56.9	57.7	821-825 bp	1.0	45.5
ndhK_p4	TCCCCCTTTCTTATTCTGTACA	TGGCGAAAAGGAGCATTAGA	40.9	45.0	56.2	57.2	805-809 bp	1.0	43.6
ndhK_p5	CCCCCTTTCTTATTCTGTACA	GGTTCAGTTTATGCTTGGCG	42.9	50.0	54.6	57.7	819-823 bp	1.0	43.3