Hotspot

ndhE-ndhG

SSC · 119756-120007 · 252 bp

Back to Eriostemon

Pi 0.0159

MarkerSeek score 61.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 252
variable_sites: 4
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AGTTCAAGACACATCAGGGC	TTGATAGGTGCAATTGCCGT	50.0	45.0	57.8	58.2	392 bp	1.0	78.0
ndhE-ndhG_p2	CGAACCATATTTCGGCTCGT	TTGATAGGTGCAATTGCCGT	50.0	45.0	58.4	58.2	371 bp	1.0	76.1
ndhE-ndhG_p3	CAGGGCCCGAACCATATTTC	TTGATAGGTGCAATTGCCGT	55.0	45.0	58.7	58.2	378 bp	1.0	74.1
ndhE-ndhG_p4	GTTCAAGACACATCAGGGCC	TTGATAGGTGCAATTGCCGT	55.0	45.0	58.8	58.2	391 bp	1.0	72.8
ndhE-ndhG_p5	ATCAGGGCCCGAACCATATT	TTGATAGGTGCAATTGCCGT	50.0	45.0	58.9	58.2	380 bp	1.0	72.7