Hotspot

trnN-GUU-rpl32

SSC · 110794-115018 · 4225 bp

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Pi 0.00569
MarkerSeek score 48.10
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
4225
variable_sites
24
pi
0.00569
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 TGCCATTCTTGCATATTTCGA TGGAACTGCCATTCAAAAA 38.1 36.8 56.3 53.2 178-179 bp 1.0 41.8
trnN-GUU-rpl32_p2 TGCCATTCTTGCATATTTCGAT TGGAACTGCCATTCAAAAA 36.4 36.8 56.8 53.2 178-179 bp 1.0 41.8
trnN-GUU-rpl32_p3 TGCCATTCTTGCATATTTCG TGGAACTGCCATTCAAAAA 40.0 36.8 54.7 53.2 178-179 bp 1.0 41.8
trnN-GUU-rpl32_p4 TGCCATTCTTGCATATTTCGATT TGGAACTGCCATTCAAAAA 34.8 36.8 57.5 53.2 178-179 bp 1.0 41.8
trnN-GUU-rpl32_p5 GCCATTCTTGCATATTTCGA TGGAACTGCCATTCAAAAA 40.0 36.8 54.4 53.2 177-178 bp 1.0 41.8