Hotspot

rps3-rpl22

LSC · 84928-85223 · 296 bp

Back to Eriostemon

Pi 0.0211

MarkerSeek score 53.50

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 296
variable_sites: 6
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CAATTCCCTCAAGACCGGAG	TCCCGGGCATCTACCATTAT	55.0	50.0	58.0	58.0	870-873 bp	1.0	81.6
rps3-rpl22_p2	CGCCCTGCAATTTGTACTTG	TCCCGGGCATCTACCATTAT	50.0	50.0	58.0	58.0	1189-1192 bp	1.0	81.1
rps3-rpl22_p3	GCGCAATTTCTTTTCCGTCA	TCCCGGGCATCTACCATTAT	45.0	50.0	58.0	58.0	1212-1215 bp	1.0	80.8
rps3-rpl22_p4	CGCCCTGCAATTTGTACTTG	GCCGCCGTACTAAATAGGAG	50.0	55.0	58.0	57.9	1383-1386 bp	1.0	80.7
rps3-rpl22_p5	CGCCCTGCAATTTGTACTTG	GGAAGGGCTCCAATTGGTAG	50.0	55.0	58.0	57.9	1489-1492 bp	1.0	80.6