Hotspot

petN-psbM

LSC · 29468-30743 · 1276 bp

Back to Eriostemon

Pi 0.0125

MarkerSeek score 61.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1276
variable_sites: 16
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACATTTTCCCTTTCGCTCGT	TCGTTCTAGTTCCTACTGCCT	45.0	47.6	58.1	57.9	1386-1398 bp	1.0	71.4
petN-psbM_p2	CCCTTTCGCTCGTAGTATGG	TCGTTCTAGTTCCTACTGCCT	55.0	47.6	57.9	57.9	1379-1391 bp	1.0	71.2
petN-psbM_p3	CCTTTCGCTCGTAGTATGGG	TCGTTCTAGTTCCTACTGCCT	55.0	47.6	57.9	57.9	1378-1390 bp	1.0	71.2
petN-psbM_p4	CTTTCGCTCGTAGTATGGGG	TCGTTCTAGTTCCTACTGCCT	55.0	47.6	57.9	57.9	1377-1389 bp	1.0	71.2
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TCGTTCTAGTTCCTACTGCCT	50.0	47.6	58.2	57.9	1424-1436 bp	1.0	71.1