Hotspot

trnK-UUU-rps16

LSC · 4448-5033 · 586 bp

Back to Eriostemon

Pi 0.0189

MarkerSeek score 69.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 586
variable_sites: 11
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGCCGTACTTAAAAGCCGAG	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.0	58.0	682-718 bp	1.0	81.5
trnK-UUU-rps16_p2	CTCTCCCGAAGATTTGGACG	ATTTCAAAGAAAGCGGGGGT	55.0	45.0	58.1	58.0	736-772 bp	1.0	80.8
trnK-UUU-rps16_p3	AGCCGTACTTAAAAGCCGAG	CCAACCTACAGGAACCGTTC	50.0	55.0	58.0	58.2	707-743 bp	1.0	79.9
trnK-UUU-rps16_p4	AGCCGTACTTAAAAGCCGAG	TCAAAGAAAGCGGGGGTTTT	50.0	45.0	58.0	58.2	679-715 bp	1.0	79.8
trnK-UUU-rps16_p5	AGCCGTACTTAAAAGCCGAG	TTCAAAGAAAGCGGGGGTTT	50.0	45.0	58.0	58.2	680-716 bp	1.0	79.8