Hotspot

trnT-UGU

LSC · 15381-15453 · 73 bp

Back to Eriosema

Pi 0.0137

MarkerSeek score 60.40

Primer available yes

Label trnT-UGU

Feature Metadata

label_name: trnT-UGU
feature_type: tRNA
region: LSC
length_bp: 73
variable_sites: 1
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU_p1	CACGCAATTTTGGCTACGTT	TGTTCCACAGAGATCGAACTC	45.0	47.6	58.0	57.4	177 bp	1.0	67.5
trnT-UGU_p2	CACGCAATTTTGGCTACGTT	TGTTCCACAGAGATCGAACT	45.0	45.0	58.0	56.2	177 bp	1.0	65.8
trnT-UGU_p3	CACGCAATTTTGGCTACGTT	GTTCCACAGAGATCGAACTCT	45.0	47.6	58.0	57.1	176 bp	1.0	65.2
trnT-UGU_p4	CACGCAATTTTGGCTACGTT	TCCACAGAGATCGAACTCTC	45.0	50.0	58.0	56.1	174 bp	1.0	65.0
trnT-UGU_p5	CACGCAATTTTGGCTACGTT	TTGTTCCACAGAGATCGAACT	45.0	42.9	58.0	57.0	178 bp	1.0	63.9