Hotspot

ndhA

SSC · 118594-120872 · 2279 bp

Back to Eriosema

Pi 0.00132

MarkerSeek score 57.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2279
variable_sites: 3
pi: 0.00132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CTCGGACTGTTTGTTGACCA	TGGGAGAAGTTGATCGTTGA	50.0	45.0	58.1	56.5	2417-2419 bp	1.0	67.6
ndhA_p2	CTCGGACTGTTTGTTGACCA	TGGGAGAAGTTGATCGTTGAA	50.0	42.9	58.1	57.2	2417-2419 bp	1.0	65.6
ndhA_p3	AGCTCGGACTGTTTGTTGAC	TGGGAGAAGTTGATCGTTGA	50.0	45.0	58.4	56.5	2419-2421 bp	1.0	64.7
ndhA_p4	CAGCTCGGACTGTTTGTTGA	TGGGAGAAGTTGATCGTTGA	50.0	45.0	58.4	56.5	2420-2422 bp	1.0	64.7
ndhA_p5	CTCGGACTGTTTGTTGACCA	ATGGGAGAAGTTGATCGTTGA	50.0	42.9	58.1	57.0	2418-2420 bp	1.0	63.8