Hotspot

trnP-UGG-psaJ

LSC · 66230-66506 · 277 bp

Back to Eriosema

Pi 0.00361
MarkerSeek score 42.30
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
277
variable_sites
1
pi
0.00361
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC TGGTGCCACGGAAAGATATG 50.0 50.0 58.1 58.0 368 bp 1.0 79.1
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA TGGTGCCACGGAAAGATATG 50.0 50.0 58.1 58.0 367 bp 1.0 79.1
trnP-UGG-psaJ_p3 ACCCGTGACATTTTGTACCC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 367 bp 1.0 79.1
trnP-UGG-psaJ_p4 CCCGTGACATTTTGTACCCA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 366 bp 1.0 79.1
trnP-UGG-psaJ_p5 ACAGGATTTGAACCCGTGAC TGGTGCCACGGAAAGATATG 50.0 50.0 58.1 58.0 379 bp 1.0 79.1