Hotspot

petA-psbJ

LSC · 62484-63348 · 865 bp

Back to Eriosema

Pi 0.00116

MarkerSeek score 59.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 865
variable_sites: 1
pi: 0.00116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTGTTGTT	CATATTCCGGATTGGGCTCA	50.0	50.0	58.3	57.4	1005 bp	1.0	72.9
petA-psbJ_p2	GATCCGTTACGTGTCCAAGG	CATATTCCGGATTGGGCTCA	55.0	50.0	58.4	57.4	1014 bp	1.0	72.6
petA-psbJ_p3	TACGTGTCCAAGGTCTGTTG	CATATTCCGGATTGGGCTCA	50.0	50.0	57.5	57.4	1007 bp	1.0	71.4
petA-psbJ_p4	GTTACGTGTCCAAGGTCTGT	CATATTCCGGATTGGGCTCA	50.0	50.0	57.5	57.4	1009 bp	1.0	71.4
petA-psbJ_p5	CGTTACGTGTCCAAGGTCTG	CATATTCCGGATTGGGCTCA	55.0	50.0	58.6	57.4	1010 bp	1.0	70.9