Hotspot

atpI-atpH

LSC · 47197-48210 · 1014 bp

Back to Eriosema

Pi 0.00197

MarkerSeek score 59.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 2
pi: 0.00197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCTGCGGCTTATATAGGTGA	CCAATAGACGCAAGCCCTAC	50.0	55.0	57.3	58.4	1124 bp	1.0	70.9
atpI-atpH_p2	GCTGCGGCTTATATAGGTGA	CAACCCCAGGTCCAATAGAC	50.0	55.0	57.3	57.6	1135 bp	1.0	70.8
atpI-atpH_p3	GCTGCGGCTTATATAGGTGA	AATCCCTTCTACAGCTTGCC	50.0	50.0	57.3	57.6	1173 bp	1.0	70.8
atpI-atpH_p4	ACAAGCGGTATTCAAGCTCT	CCAATAGACGCAAGCCCTAC	45.0	55.0	57.2	58.4	1160 bp	1.0	70.6
atpI-atpH_p5	ACAAGCGGTATTCAAGCTCT	CAACCCCAGGTCCAATAGAC	45.0	55.0	57.2	57.6	1171 bp	1.0	70.6