Hotspot

trnC-GCA-rpoB

LSC · 33929-34765 · 837 bp

Back to Eriosema

Pi 0.00239

MarkerSeek score 60.10

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 837
variable_sites: 2
pi: 0.00239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CCGGATTTGAACTGGGGAAA	CCTTCATTTCCACCCCCAAG	50.0	55.0	58.1	58.4	922-945 bp	1.0	76.0
trnC-GCA-rpoB_p2	CCGGATTTGAACTGGGGAAA	ATTCCTTCATTTCCACCCCC	50.0	50.0	58.1	57.4	925-948 bp	1.0	75.1
trnC-GCA-rpoB_p3	AAAAGGATTTGCAGTCCCCC	CCTTCATTTCCACCCCCAAG	50.0	55.0	58.4	58.4	904-927 bp	1.0	73.8
trnC-GCA-rpoB_p4	AAAAGGATTTGCAGTCCCCC	ATTCCTTCATTTCCACCCCC	50.0	50.0	58.4	57.4	907-930 bp	1.0	72.9
trnC-GCA-rpoB_p5	CCGGATTTGAACTGGGGAAA	TCCTTCATTTCCACCCCCAA	50.0	50.0	58.1	58.8	923-946 bp	1.0	72.8