Hotspot

petN-trnC-GCA

LSC · 33057-33857 · 801 bp

Back to Eriosema

Pi 0.00125

MarkerSeek score 59.50

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 1
pi: 0.00125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.0	58.1	864-865 bp	1.0	79.4
petN-trnC-GCA_p2	GCCCAAGCAAGACTTACGAT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	58.3	58.1	856-857 bp	1.0	77.3
petN-trnC-GCA_p3	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.0	58.4	882-883 bp	1.0	77.2
petN-trnC-GCA_p4	TCTTCCCCATACTACGAGGG	TTTCCCCAGTTCAAATCCGG	55.0	50.0	57.6	58.1	908-909 bp	1.0	76.4
petN-trnC-GCA_p5	TTAAAGCAGCCCAAGCAAGA	TATCTATTTGGCGGCATGGC	45.0	50.0	58.0	58.5	915-916 bp	1.0	76.3