Hotspot

psbM-petN

LSC · 32202-32966 · 765 bp

Back to Eriosema

Pi 0.00261

MarkerSeek score 60.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 2
pi: 0.00261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCATTGTTTATTCT	TCTTGCTTGGGCTGCTTTAA	33.3	45.0	53.5	58.0	920 bp	1.0	40.2
psbM-petN_p2	ACGTAAAAACGGTAAGTAAAAGTGA	TCTTGCTTGGGCTGCTTTAA	32.0	45.0	57.5	58.0	870 bp	1.0	40.2
psbM-petN_p3	TGCTACTGCATTGTTTATTCTAGT	TCTTGCTTGGGCTGCTTTAA	33.3	45.0	56.4	58.0	920 bp	1.0	40.2
psbM-petN_p4	ACTGCATTGTTTATTCTAGTTCCT	TCTTGCTTGGGCTGCTTTAA	33.3	45.0	56.4	58.0	916 bp	1.0	40.2
psbM-petN_p5	TGCTACTGCATTGTTTATTCT	ATCGTAAGTCTTGCTTGGGC	33.3	50.0	53.5	58.3	928 bp	1.0	40.2