Hotspot

trnT-GGU-trnE-UUC

LSC · 30165-30945 · 781 bp

Back to Eriosema

Pi 0.00256

MarkerSeek score 57.10

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 2
pi: 0.00256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.3	884 bp	1.0	78.0
trnT-GGU-trnE-UUC_p2	GAACCGATGACTTACGCCTT	TGTCCTGAACCACTAGACGA	50.0	50.0	58.0	57.7	863 bp	1.0	77.9
trnT-GGU-trnE-UUC_p3	CCATGGCGTTACTCTACCAC	GCTGCCTCCTTGAAAGAGAG	55.0	55.0	58.1	58.3	863 bp	1.0	77.6
trnT-GGU-trnE-UUC_p4	CCATGGCGTTACTCTACCAC	TGTCCTGAACCACTAGACGA	55.0	50.0	58.1	57.7	842 bp	1.0	77.4
trnT-GGU-trnE-UUC_p5	TGGCGTTACTCTACCACTGA	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.1	58.3	860 bp	1.0	77.4