Hotspot

ycf2

IRb · 89682-96512 · 6831 bp

Back to Eriolarynx

Pi 0.000878

MarkerSeek score 40.60

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6831
variable_sites: 9
pi: 0.000878
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ATTCCGCCAAGATCTCTTCG	TCGTTCCGTTTGAAGAAAGGA	50.0	42.9	57.8	57.8	358 bp	1.0	70.0
ycf2_p2	TCTCTTCGTTAGTTGGGGGA	TCGTTCCGTTTGAAGAAAGGA	50.0	42.9	57.7	57.8	346 bp	1.0	69.4
ycf2_p3	GCAATTCCGCCAAGATCTCT	TCGTTCCGTTTGAAGAAAGGA	50.0	42.9	58.3	57.8	361 bp	1.0	69.3
ycf2_p4	CCGCCAAGATCTCTTCGTTA	TCGTTCCGTTTGAAGAAAGGA	50.0	42.9	57.4	57.8	355 bp	1.0	67.3
ycf2_p5	ATTCCGCCAAGATCTCTTCG	GGAAGGATCCCAAAGAATCGA	50.0	47.6	57.8	57.4	340 bp	1.0	67.1