Hotspot

rpoC2

LSC · 17126-21304 · 4179 bp

Back to Eriolarynx

Pi 0.000479

MarkerSeek score 54.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4179
variable_sites: 3
pi: 0.000479
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CTCCAATCGATTGACCTGCA	GGATGATGCCGGAAAGGATT	50.0	50.0	58.0	58.0	285 bp	1.0	80.2
rpoC2_p2	AGGACTCCGGCCATAACATA	GGATGATGCCGGAAAGGATT	50.0	50.0	57.9	58.0	221 bp	1.0	79.8
rpoC2_p3	TAGGACTCCGGCCATAACAT	GGATGATGCCGGAAAGGATT	50.0	50.0	57.9	58.0	222 bp	1.0	79.8
rpoC2_p4	CTCCAATCGATTGACCTGCA	AGTCCTCGGAATGGGATGAT	50.0	50.0	58.0	57.9	298 bp	1.0	79.4
rpoC2_p5	CTCCAATCGATTGACCTGCA	GTCCTCGGAATGGGATGATG	50.0	55.0	58.0	57.8	297 bp	1.0	78.9