Hotspot

rpl16

LSC · 84476-85922 · 1447 bp

Back to Eriolarynx

Pi 0.000921

MarkerSeek score 60.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1447
variable_sites: 2
pi: 0.000921
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AACATGCGGTTTTGTTTCAT	TCAATCAAGGGAAACATTTCT	35.0	33.3	55.0	53.3	1563-1580 bp	1.0	41.0
rpl16_p2	AACATGCGGTTTTGTTTCAT	ATCAATCAAGGGAAACATTTCT	35.0	31.8	55.0	53.9	1564-1581 bp	1.0	41.0
rpl16_p3	AACATGCGGTTTTGTTTCAT	AATCAATCAAGGGAAACATTTCT	35.0	30.4	55.0	54.7	1565-1582 bp	1.0	41.0
rpl16_p4	ACATGCGGTTTTGTTTCATATCT	TCAATCAAGGGAAACATTTCT	34.8	33.3	57.4	53.3	1562-1579 bp	1.0	41.0
rpl16_p5	AACATGCGGTTTTGTTTCAT	TCGATTGACCATTTGATATAATTGCT	35.0	30.8	55.0	57.6	1511-1528 bp	1.0	41.0