Hotspot

rpl32-trnL-UAG

SSC · 116287-117095 · 809 bp

Back to Eriolarynx

Pi 0.000824

MarkerSeek score 62.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 809
variable_sites: 1
pi: 0.000824
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGAAGGGATATTGGGTAGCA	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.9	57.9	953-969 bp	1.0	72.1
rpl32-trnL-UAG_p2	AGGAAGGGATATTGGGTAGCA	TCGAACCGAGATGCATTAGC	47.6	50.0	57.9	58.1	979-995 bp	1.0	71.8
rpl32-trnL-UAG_p3	AGGAAGGGATATTGGGTAGCA	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.9	57.8	946-962 bp	1.0	71.6
rpl32-trnL-UAG_p4	AGGAAGGGATATTGGGTAGCA	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.9	57.8	952-968 bp	1.0	71.5
rpl32-trnL-UAG_p5	AGGAAGGGATATTGGGTAGCA	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.9	57.8	951-967 bp	1.0	71.5