Hotspot

trnM-CAU-atpE

LSC · 54870-55090 · 221 bp

Back to Eriolarynx

Pi 0.00302

MarkerSeek score 57.00

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 221
variable_sites: 1
pi: 0.00302
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	AGTACTGCTTTCATACGGCG	GGCAAATCTAGCTCTCCGAC	50.0	55.0	58.1	58.1	328-333 bp	1.0	79.7
trnM-CAU-atpE_p2	AGTACTGCTTTCATACGGCG	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.1	57.9	330-335 bp	1.0	79.6
trnM-CAU-atpE_p3	AGTACTGCTTTCATACGGCG	AGGCAAATCTAGCTCTCCGA	50.0	50.0	58.1	58.2	329-334 bp	1.0	79.1
trnM-CAU-atpE_p4	AGTACTGCTTTCATACGGCG	AAGGCTGAAGGAAGGAGACA	50.0	50.0	58.1	58.3	357-362 bp	1.0	78.6
trnM-CAU-atpE_p5	AGTACTGCTTTCATACGGCG	AGGCTGAAGGAAGGAGACAA	50.0	50.0	58.1	58.3	356-361 bp	1.0	78.6