Hotspot

ndhC-trnV-UAC

LSC · 52841-53954 · 1114 bp

Back to Eriolarynx

Pi 0.0018
MarkerSeek score 60.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1114
variable_sites
3
pi
0.0018
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTGCTAATCGGGGCTAACAC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.0 57.8 1253-1255 bp 1.0 78.4
ndhC-trnV-UAC_p2 GCTAATCGGGGCTAACACTC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.1 57.8 1251-1253 bp 1.0 77.6
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1256-1258 bp 1.0 75.9
ndhC-trnV-UAC_p4 TTGCTAATCGGGGCTAACAC GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.0 57.5 1252-1254 bp 1.0 75.7
ndhC-trnV-UAC_p5 GGCTAACACTCCGGAAATGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.6 57.8 1242-1244 bp 1.0 75.5