Hotspot

psaA-ycf3

LSC · 43503-44342 · 840 bp

Back to Eriolarynx

Pi 0

MarkerSeek score 61.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	929-1018 bp	1.0	82.7
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	911-1000 bp	1.0	82.7
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CAGAGGCTTGGTTCGATCAA	50.0	50.0	57.9	57.9	944-1033 bp	1.0	82.3
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	931-1020 bp	1.0	81.9
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	925-1014 bp	1.0	81.0