Hotspot

lhbA-trnG-UCC

LSC · 37810-38086 · 277 bp

Back to Eriolarynx

Pi 0

MarkerSeek score 24.20

Primer available yes

Label lhbA-trnG-UCC

Feature Metadata

label_name: lhbA-trnG-UCC
feature_type: igs
region: LSC
length_bp: 277
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 24.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
lhbA-trnG-UCC_p1	TCCTGATGGCTGGTCAAGTA	AATCGAACCCGCATCTTCTC	50.0	50.0	58.0	58.1	438 bp	1.0	79.1
lhbA-trnG-UCC_p2	TCCTGATGGCTGGTCAAGTA	GAATCGAACCCGCATCTTCT	50.0	50.0	58.0	58.1	439 bp	1.0	79.1
lhbA-trnG-UCC_p3	TTTGCTTCTCCTGATGGCTG	AATCGAACCCGCATCTTCTC	50.0	50.0	58.2	58.1	446 bp	1.0	78.2
lhbA-trnG-UCC_p4	TTTGCTTCTCCTGATGGCTG	GAATCGAACCCGCATCTTCT	50.0	50.0	58.2	58.1	447 bp	1.0	78.2
lhbA-trnG-UCC_p5	TCCTGATGGCTGGTCAAGTA	GAACCCGCATCTTCTCCTTG	50.0	55.0	58.0	58.6	434 bp	1.0	74.6