Hotspot

trnC-GCA-petN

LSC · 28910-29582 · 673 bp

Back to Eriolarynx

Pi 0.00198

MarkerSeek score 60.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 2
pi: 0.00198
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	735-736 bp	1.0	79.6
trnC-GCA-petN_p2	GTAGCATTTTGGCGACATGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.8	58.0	788-789 bp	1.0	78.7
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	729-730 bp	1.0	78.1
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CCTAGAGTCCACTCCTTCCC	50.0	60.0	58.1	58.2	792-793 bp	1.0	77.9
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	CTAGAGTCCACTCCTTCCCC	50.0	60.0	58.1	58.2	791-792 bp	1.0	77.9