Hotspot

rpoC1

LSC · 24649-26700 · 2052 bp

Back to Eriochrysis

Pi 0.000975

MarkerSeek score 58.20

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2052
variable_sites: 2
pi: 0.000975
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AGGGAGGATGTTTGATCGGA	ACTAGCGAAATCGGAATGCC	50.0	50.0	58.1	58.4	2160 bp	1.0	75.8
rpoC1_p2	AGGGAGGATGTTTGATCGGA	TCTCAATCTGGATTTGCGCG	50.0	50.0	58.1	59.0	2194 bp	1.0	71.2
rpoC1_p3	AGGGAGGATGTTTGATCGGA	TAGCGAAATCGGAATGCCC	50.0	52.6	58.1	58.0	2158 bp	1.0	71.1
rpoC1_p4	GGGAGGATGTTTGATCGGAA	ACTAGCGAAATCGGAATGCC	50.0	50.0	57.3	58.4	2159 bp	1.0	71.1
rpoC1_p5	AGGGAGGATGTTTGATCGGA	CTAGCGAAATCGGAATGCCC	50.0	55.0	58.1	59.1	2159 bp	1.0	70.1