Hotspot

atpA

LSC · 36558-38081 · 1524 bp

Back to Eriochrysis

Pi 0

MarkerSeek score 31.30

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	ATTCTCGGGGCCATAGAATG	ACGATTCCTCTTCTACTAACACG	50.0	43.5	57.1	57.8	1698 bp	1.0	47.2
atpA_p2	ATTCTCGGGGCCATAGAATG	CGATTCCTCTTCTACTAACACGA	50.0	43.5	57.1	57.5	1697 bp	1.0	45.2
atpA_p3	ATTCTCGGGGCCATAGAATG	TCTTTCTCAACGATTCCTCTTCT	50.0	39.1	57.1	57.3	1707 bp	1.0	43.2
atpA_p4	ATTCTCGGGGCCATAGAATG	TCCTCTTCTACTAACACGAGT	50.0	42.9	57.1	55.2	1693 bp	1.0	42.8
atpA_p5	ATTCTCGGGGCCATAGAATG	CGATTCCTCTTCTACTAACACG	50.0	45.5	57.1	56.1	1697 bp	1.0	42.2