Hotspot

ycf15-trnI-CAU

IRa · 135272-137824 · 2553 bp

Back to Eriochrysis

Pi 0
MarkerSeek score 56.70
Primer available yes
Label ycf15-trnI-CAU

Feature Metadata

label_name
ycf15-trnI-CAU
feature_type
igs
region
IRa
length_bp
2553
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnI-CAU_p1 TTCAGGGCCTATCCGAAAGA GTTGGGCGCTTTAACCATTC 50.0 50.0 58.1 58.0 2691-2696 bp 1.0 79.1
ycf15-trnI-CAU_p2 CTCCGCGGATATCTTTGCTT GTTGGGCGCTTTAACCATTC 50.0 50.0 58.1 58.0 2620-2625 bp 1.0 79.0
ycf15-trnI-CAU_p3 GCGGATATCTTTGCTTCGGA GTTGGGCGCTTTAACCATTC 50.0 50.0 58.1 58.0 2616-2621 bp 1.0 79.0
ycf15-trnI-CAU_p4 TCCGCGGATATCTTTGCTTC GTTGGGCGCTTTAACCATTC 50.0 50.0 58.1 58.0 2619-2624 bp 1.0 79.0
ycf15-trnI-CAU_p5 TTCAGGGCCTATCCGAAAGA GAGCCAGTTCCAATAGACCG 50.0 55.0 58.1 58.1 2765-2770 bp 1.0 78.7