Hotspot

ndhB-trnL-CAA

IRa · 133541-134116 · 576 bp

Back to Eriochrysis

Pi 0

MarkerSeek score 58.30

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 576
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	509-636 bp	1.0	83.5
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	507-634 bp	1.0	83.5
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	504-631 bp	1.0	82.0
ndhB-trnL-CAA_p4	CCCCATTCTTGCAATTGCTC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.7	58.0	518-645 bp	1.0	81.5
ndhB-trnL-CAA_p5	CCCCATTCTTGCAATTGCTC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.7	58.0	516-643 bp	1.0	81.5