Hotspot

rps11-rpl36

LSC · 77693-77866 · 174 bp

Back to Eriochrysis

Pi 0

MarkerSeek score 33.80

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGCAAACCAATACGCACTTT	CGTGGGCGAATTAGAGTCAT	45.0	50.0	58.0	57.8	287 bp	1.0	77.8
rps11-rpl36_p2	CTACGCAAACCAATACGCAC	CGTGGGCGAATTAGAGTCAT	50.0	50.0	57.8	57.8	290 bp	1.0	77.0
rps11-rpl36_p3	TCTACGTGCACTCTTCCGTA	CGTGGGCGAATTAGAGTCAT	50.0	50.0	58.2	57.8	327 bp	1.0	76.7
rps11-rpl36_p4	TACGCAAACCAATACGCACT	CGTGGGCGAATTAGAGTCAT	45.0	50.0	58.2	57.8	289 bp	1.0	76.6
rps11-rpl36_p5	CGTGCACTCTTCCGTAAACT	CGTGGGCGAATTAGAGTCAT	50.0	50.0	58.2	57.8	323 bp	1.0	76.4