Hotspot

ycf4-cemA

LSC · 60328-60694 · 367 bp

Back to Eriochrysis

Pi 0.00272

MarkerSeek score 55.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 1
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AGAGAAAGAGACTCCCCAGG	45.0	55.0	58.1	57.8	484 bp	1.0	77.4
ycf4-cemA_p2	ACAAAAAGCTGCCGAATTGG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.8	57.8	500 bp	1.0	77.0
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	CCCAGGGCAAAAGTACGATA	45.0	50.0	58.1	57.3	470 bp	1.0	73.8
ycf4-cemA_p4	ACAAAAAGCTGCCGAATTGG	CCCAGGGCAAAAGTACGATA	45.0	50.0	57.8	57.3	486 bp	1.0	73.3
ycf4-cemA_p5	TTGGCCTATTTCTTGCGTGT	GAGAAAGAGACTCCCCAGGG	45.0	60.0	58.1	58.8	483 bp	1.0	73.0