Hotspot

atpA-trnR-UCU

LSC · 38082-38218 · 137 bp

Back to Eriochrysis

Pi 0.0073

MarkerSeek score 56.70

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 1
pi: 0.0073
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	AGGAACAGCTTGAACGGTTT	AAGAGAAGCGTCCATTGTCT	45.0	45.0	57.9	56.9	257 bp	1.0	70.8
atpA-trnR-UCU_p2	AGCTTGAACGGTTTTCCCTT	AAGAGAAGCGTCCATTGTCT	45.0	45.0	57.9	56.9	251 bp	1.0	70.7
atpA-trnR-UCU_p3	ATTCACCGAGCAAGCAGAAA	AAGAGAAGCGTCCATTGTCT	45.0	45.0	58.1	56.9	298 bp	1.0	70.3
atpA-trnR-UCU_p4	AGGAACAGCTTGAACGGTTT	AAAGAGAAGCGTCCATTGTCT	45.0	42.9	57.9	57.6	258 bp	1.0	68.6
atpA-trnR-UCU_p5	AGGAACAGCTTGAACGGTTT	AGAAAGAGAAGCGTCCATTGT	45.0	42.9	57.9	57.6	260 bp	1.0	68.6