Hotspot

ndhF

SSC · 104561-106777 · 2217 bp

Back to Eriochloa

Pi 0.00902

MarkerSeek score 48.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 30
pi: 0.00902
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGCATTTTGATGAGCCTCGT	AGAGTGGGTTTGGTGAATCG	45.0	50.0	58.2	57.8	2352 bp	1.0	78.3
ndhF_p2	TGCATTTTGATGAGCCTCGT	TGGGTTTGGTGAATCGGAAA	45.0	45.0	58.2	57.6	2348 bp	1.0	76.9
ndhF_p3	TGCATTTTGATGAGCCTCGT	GTGGGTTTGGTGAATCGGAA	45.0	50.0	58.2	58.4	2349 bp	1.0	76.7
ndhF_p4	TTCGAGCCGAAAGATGGATC	TGGGTTTGGTGAATCGGAAA	50.0	45.0	57.8	57.6	2998 bp	0.666667	62.1
ndhF_p5	TGCATTTTGATGAGCCTCGT	TGGTATGGGGGATAAGCTCC	45.0	55.0	58.2	58.3	2871 bp	0.333333	49.9