Hotspot

ccsA

SSC · 108504-109478 · 975 bp

Back to Eriochloa

Pi 0.0164

MarkerSeek score 59.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 975
variable_sites: 24
pi: 0.0164
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGCATTCTCGAAAAAGAA	CAAGGGGTTCTCAAATCAAACA	45.0	40.9	57.4	57.1	1128 bp	1.0	53.3
ccsA_p2	GCGGCATTCTCGAAAAAGAA	TCAAGGGGTTCTCAAATCAAAC	45.0	40.9	57.4	56.8	1129 bp	1.0	51.2
ccsA_p3	GCGGCATTCTCGAAAAAGAA	AGGGGTTCTCAAATCAAACA	45.0	40.0	57.4	54.7	1126 bp	1.0	50.7
ccsA_p4	GCGGCATTCTCGAAAAAGAA	CAAGGGGTTCTCAAATCAAACAA	45.0	39.1	57.4	57.7	1128 bp	1.0	50.6
ccsA_p5	GCGGCATTCTCGAAAAAGAA	AGGGGTTCTCAAATCAAACAA	45.0	38.1	57.4	55.6	1126 bp	1.0	49.5