Hotspot

psaC-ndhE

SSC · 111544-112029 · 486 bp

Back to Eriochloa

Pi 0.0194

MarkerSeek score 69.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 486
variable_sites: 14
pi: 0.0194
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GTTGGACAAGCTCGTACACA	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.1	56.4	648-669 bp	1.0	70.3
psaC-ndhE_p2	TGTTGGACAAGCTCGTACAC	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.1	56.4	649-670 bp	1.0	70.3
psaC-ndhE_p3	CTGTTGGACAAGCTCGTACA	TTTTGTTATAGCCCTTGCGG	50.0	45.0	57.9	56.4	650-671 bp	1.0	70.2
psaC-ndhE_p4	ACATCTGTTGGACAAGCTCG	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.2	56.4	654-675 bp	1.0	69.8
psaC-ndhE_p5	CATCTGTTGGACAAGCTCGT	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.2	56.4	653-674 bp	1.0	69.8