Hotspot

rpl36-infA

LSC · 77615-77714 · 100 bp

Back to Eriochloa

Pi 0.0267

MarkerSeek score 63.70

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 100
variable_sites: 4
pi: 0.0267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	TGACTCTAATTCGTCCACGC	ATTCGAAGCGTACCGAAGAC	50.0	50.0	57.7	58.1	235-244 bp	1.0	78.7
rpl36-infA_p2	TGACTCTAATTCGTCCACGC	TTATGATTCCAGCAAGGGGC	50.0	50.0	57.7	57.9	282-291 bp	1.0	78.6
rpl36-infA_p3	TGACTCTAATTCGTCCACGC	GATTCGAAGCGTACCGAAGA	50.0	50.0	57.7	57.8	236-245 bp	1.0	78.0
rpl36-infA_p4	TGACTCTAATTCGTCCACGC	CCCATAAGGATTCGAAGCGT	50.0	50.0	57.7	57.8	244-253 bp	1.0	77.5
rpl36-infA_p5	CTCTAATTCGTCCACGCCTG	TTATGATTCCAGCAAGGGGC	55.0	50.0	58.4	57.9	279-288 bp	1.0	77.3