Hotspot

rpl20-rps12

LSC · 68189-68894 · 706 bp

Back to Eriochloa

Pi 0.00803

MarkerSeek score 63.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 8
pi: 0.00803
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACGCATTTTTGTTCGTCGTC	GAGGAACATGTGCTAGGGTG	45.0	55.0	58.0	58.0	768-783 bp	1.0	82.0
rpl20-rps12_p2	ACGCATTTTTGTTCGTCGTC	CGACATGCCAACGGTTAAAC	45.0	50.0	58.0	58.0	863-878 bp	1.0	81.7
rpl20-rps12_p3	ACGCATTTTTGTTCGTCGTC	GTCGAGGAACATGTGCTAGG	45.0	55.0	58.0	58.1	771-786 bp	1.0	81.7
rpl20-rps12_p4	GTTTGAGGCAAATGAACGCA	GAGGAACATGTGCTAGGGTG	45.0	55.0	57.9	58.0	783-798 bp	1.0	81.0
rpl20-rps12_p5	GTTTGAGGCAAATGAACGCA	CGACATGCCAACGGTTAAAC	45.0	50.0	57.9	58.0	878-893 bp	1.0	80.7