Hotspot

petL-petG

LSC · 65083-65261 · 179 bp

Back to Eriochloa

Pi 0.0187

MarkerSeek score 64.60

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 179
variable_sites: 5
pi: 0.0187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TGGTTTTCTACTGGCTGCTT	ACGCAGTCACGAATAATCCC	45.0	50.0	57.3	58.1	327-328 bp	1.0	75.7
petL-petG_p2	TTGGTTTTCTACTGGCTGCT	ACGCAGTCACGAATAATCCC	45.0	50.0	57.3	58.1	328-329 bp	1.0	75.7
petL-petG_p3	TGGTTTTCTACTGGCTGCTT	CAGTCACGAATAATCCCGCT	45.0	50.0	57.3	57.8	324-325 bp	1.0	74.4
petL-petG_p4	TTGGTTTTCTACTGGCTGCT	CAGTCACGAATAATCCCGCT	45.0	50.0	57.3	57.8	325-326 bp	1.0	74.4
petL-petG_p5	TGGTTTTCTACTGGCTGCTT	CGCCTGTATTGCAAATACGC	45.0	50.0	57.3	58.3	343-344 bp	1.0	74.1