Hotspot

petN-trnC-GCA

LSC · 18976-19882 · 907 bp

Back to Eriochloa

Pi 0.0235

MarkerSeek score 71.00

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 32
pi: 0.0235
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGTCCACTTCTCCCCCATAC	CTGCAAATCCTTTACCCCCA	55.0	50.0	58.1	57.8	1033-1070 bp	1.0	79.7
petN-trnC-GCA_p2	AGTCCACTTCTCCCCCATAC	TGCAAATCCTTTACCCCCAG	55.0	50.0	58.1	57.8	1032-1069 bp	1.0	79.7
petN-trnC-GCA_p3	GTCCACTTCTCCCCCATACT	CTGCAAATCCTTTACCCCCA	55.0	50.0	58.1	57.8	1032-1069 bp	1.0	79.7
petN-trnC-GCA_p4	GTCCACTTCTCCCCCATACT	TGCAAATCCTTTACCCCCAG	55.0	50.0	58.1	57.8	1031-1068 bp	1.0	79.7
petN-trnC-GCA_p5	GTCCACTTCTCCCCCATACT	GCAAATCCTTTACCCCCAGT	55.0	50.0	58.1	57.8	1030-1067 bp	1.0	79.7