Hotspot

trnG-UCC-trnE-UUC

LSC · 13963-16309 · 2347 bp

Back to Eriochloa

Pi 0.0184

MarkerSeek score 61.20

Primer available yes

Label trnG-UCC-trnE-UUC

Feature Metadata

label_name: trnG-UCC-trnE-UUC
feature_type: igs
region: LSC
length_bp: 2347
variable_sites: 64
pi: 0.0184
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnE-UUC_p1	ACGAATCACACTTTTACCACT	GCTGCCTCCTTGAAAGAGAG	38.1	55.0	55.5	58.3	2428-2509 bp	1.0	52.0
trnG-UCC-trnE-UUC_p2	ACGAATCACACTTTTACCACT	TGTCCTGAACCACTAGACGA	38.1	50.0	55.5	57.7	2407-2488 bp	1.0	51.9
trnG-UCC-trnE-UUC_p3	AGAACGAATCACACTTTTACCAC	GCTGCCTCCTTGAAAGAGAG	39.1	55.0	57.3	58.3	2431-2512 bp	1.0	50.9
trnG-UCC-trnE-UUC_p4	GAACGAATCACACTTTTACCACT	GCTGCCTCCTTGAAAGAGAG	39.1	55.0	57.3	58.3	2430-2511 bp	1.0	50.9
trnG-UCC-trnE-UUC_p5	GAACGAATCACACTTTTACCACT	TGTCCTGAACCACTAGACGA	39.1	50.0	57.3	57.7	2409-2490 bp	1.0	50.8