Hotspot

trnG-GCC-trnfM-CAU

LSC · 12785-13044 · 260 bp

Back to Eriochloa

Pi 0.0256

MarkerSeek score 64.70

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 260
variable_sites: 9
pi: 0.0256
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GGCGTAGTTCAATGTAGCGA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.1	57.8	392-509 bp	1.0	83.2
trnG-GCC-trnfM-CAU_p2	GGCGTAGTTCAATGTAGCGA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.1	58.3	407-524 bp	1.0	82.7
trnG-GCC-trnfM-CAU_p3	GGCGTAGTTCAATGTAGCGA	GAGCGGAGTAGAGCAGTTTG	50.0	55.0	58.1	58.4	420-537 bp	1.0	82.0
trnG-GCC-trnfM-CAU_p4	GGCGTAGTTCAATGTAGCGA	TAGAGCGGAGTAGAGCAGTT	50.0	50.0	58.1	57.6	422-539 bp	1.0	81.5
trnG-GCC-trnfM-CAU_p5	GGCGTAGTTCAATGTAGCGA	AGAGCGGAGTAGAGCAGTTT	50.0	50.0	58.1	58.4	421-538 bp	1.0	81.3