Hotspot

rpl32-trnL-UAG

SSC · 110637-111044 · 408 bp

Back to Eriocaulon

Pi 0.0812

MarkerSeek score 78.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 408
variable_sites: 96
pi: 0.0812
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAGCCCATATGCGACGAAT	AAGAGCAGCGTGTTTACCAA	45.0	45.0	58.0	58.0	1132-1166 bp	0.571429	74.3
rpl32-trnL-UAG_p2	AAAGCCCATATGCGACGAAT	TCGAACCGAGATGCTTTAGC	45.0	50.0	58.0	58.1	1162-1196 bp	0.571429	74.0
rpl32-trnL-UAG_p3	AGCCCATATGCGACGAATAC	AAGAGCAGCGTGTTTACCAA	50.0	45.0	57.9	58.0	1130-1164 bp	0.571429	73.9
rpl32-trnL-UAG_p4	GCCCATATGCGACGAATACT	AAGAGCAGCGTGTTTACCAA	50.0	45.0	57.9	58.0	1129-1163 bp	0.571429	73.9
rpl32-trnL-UAG_p5	AAAGCCCATATGCGACGAAT	CTTCCTAAGAGCAGCGTGTT	45.0	50.0	58.0	57.9	1138-1172 bp	0.571429	73.9