Hotspot

atpB-rbcL

LSC · 52482-53387 · 906 bp

Back to Eriocaulon

Pi 0.0472

MarkerSeek score 82.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 144
pi: 0.0472
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ACGTAGAACTAGAAGTTGTAGGA	ACACCAGCTTTAAATCCAGCA	39.1	42.9	56.2	58.1	942-999 bp	1.0	52.0
atpB-rbcL_p2	ACGTAGAACTAGAAGTTGTAGGA	ACCAGCTTTAAATCCAGCACT	39.1	42.9	56.2	57.8	940-997 bp	1.0	52.0
atpB-rbcL_p3	ACGTAGAACTAGAAGTTGTAGGA	CACCAGCTTTAAATCCAGCAC	39.1	47.6	56.2	57.8	941-998 bp	1.0	52.0
atpB-rbcL_p4	ACGTAGAACTAGAAGTTGTAGGA	AGTCTCTGTTTGTGGTGACA	39.1	45.0	56.2	56.6	913-970 bp	1.0	52.0
atpB-rbcL_p5	AACGTAGAACTAGAAGTTGTAGGA	ACACCAGCTTTAAATCCAGCA	37.5	42.9	56.9	58.1	943-1000 bp	1.0	52.0