Hotspot

ndhC-trnV-UAC

LSC · 48773-49494 · 722 bp

Back to Eriocaulon

Pi 0.0619
MarkerSeek score 84.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
722
variable_sites
147
pi
0.0619
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGTCATATTTGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 33.3 55.0 57.4 57.9 577-815 bp 1.0 54.3
ndhC-trnV-UAC_p2 TGTCATATTTGTGAAGCAGAAACA CGGTTCGAATCCGTATAGCC 33.3 55.0 57.4 58.3 578-816 bp 1.0 52.5
ndhC-trnV-UAC_p3 ATGTCATATTTGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 32.0 55.0 57.8 57.9 578-816 bp 1.0 52.0
ndhC-trnV-UAC_p4 TGTCATATTTGTGAAGCAGAAACAT GGTTCGAATCCGTATAGCCC 32.0 55.0 57.8 57.9 577-815 bp 1.0 52.0
ndhC-trnV-UAC_p5 GTCATATTTGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 34.8 55.0 55.8 57.9 576-814 bp 1.0 52.0