Hotspot

trnF-GAA-ndhJ

LSC · 46398-47106 · 709 bp

Back to Eriocaulon

Pi 0.0634

MarkerSeek score 77.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 139
pi: 0.0634
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GGATTTGGAGAAGTGCCGAT	50.0	50.0	58.1	58.0	781-917 bp	1.0	91.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCGGCGGATTTTTAGCTAGT	50.0	50.0	58.1	58.1	904-1040 bp	1.0	91.2
trnF-GAA-ndhJ_p3	GGAAATCGTCGGGATAGCTC	GGATTTGGAGAAGTGCCGAT	55.0	50.0	57.9	58.0	827-963 bp	1.0	91.1
trnF-GAA-ndhJ_p4	AGTCCCTCTATCCCCACCTA	GGATTTGGAGAAGTGCCGAT	55.0	50.0	58.1	58.0	1160-1297 bp	0.857143	85.4
trnF-GAA-ndhJ_p5	AGTCCCTCTATCCCCACCTA	CCGGCGGATTTTTAGCTAGT	55.0	50.0	58.1	58.1	1283-1420 bp	0.857143	85.4