Hotspot

trnT-UGU-trnL-UAA

LSC · 44653-45314 · 662 bp

Back to Eriocaulon

Pi 0.057

MarkerSeek score 82.10

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 662
variable_sites: 133
pi: 0.057
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	57.9	58.0	679-755 bp	1.0	90.9
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	ACAATCAAGTCCGTAGCGTC	55.0	50.0	58.2	58.0	710-786 bp	1.0	90.7
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.9	57.8	664-740 bp	1.0	89.7
trnT-UGU-trnL-UAA_p4	GAGTCGAACCGATGACCATC	GCGTCTACCTATTTCGCCAT	55.0	50.0	58.2	57.8	695-771 bp	1.0	89.5
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	CAATCAAGTCCGTAGCGTCT	55.0	50.0	57.9	57.7	678-754 bp	1.0	88.7