Hotspot

trnT-GGU-psbD

LSC · 30558-31312 · 755 bp

Back to Eriocaulon

Pi 0.0512

MarkerSeek score 85.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 133
pi: 0.0512
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	871-911 bp	1.0	91.5
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	874-914 bp	1.0	91.3
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	AAACTAGGCGCAAAGAACCA	55.0	45.0	58.1	58.0	1308-1348 bp	1.0	90.3
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CGATTGGGGCAGAGAATGAA	55.0	50.0	58.1	58.0	1241-1281 bp	1.0	90.3
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	AACTAGGCGCAAAGAACCAA	55.0	45.0	58.1	58.0	1307-1347 bp	0.928571	87.3