Hotspot

atpH-atpI

LSC · 12691-13745 · 1055 bp

Back to Eriocaulon

Pi 0.0744

MarkerSeek score 82.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1055
variable_sites: 237
pi: 0.0744
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CTTCGGGTTGTCTCGCAATA	ATTAGCCGCGGCCTATATTG	50.0	50.0	58.0	58.0	835-1614 bp	1.0	91.8
atpH-atpI_p2	CTTCGGGTTGTCTCGCAATA	CATTAGCCGCGGCCTATATT	50.0	50.0	58.0	58.0	836-1615 bp	1.0	91.8
atpH-atpI_p3	AAGTCGGGTGTATCTGGGAA	ATTAGCCGCGGCCTATATTG	50.0	50.0	58.1	58.0	1100-1909 bp	0.857143	85.7
atpH-atpI_p4	AAGTCGGGTGTATCTGGGAA	CATTAGCCGCGGCCTATATT	50.0	50.0	58.1	58.0	1101-1910 bp	0.857143	85.7
atpH-atpI_p5	CTTCGGGTTGTCTCGCAATA	TATGACCCTTGGGTTCCCTT	50.0	50.0	58.0	57.9	1372-2042 bp	0.571429	74.3