Hotspot

rps16-trnQ-UUG

LSC · 5676-6061 · 386 bp

Back to Eriocaulon

Pi 0.0835
MarkerSeek score 82.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
386
variable_sites
100
pi
0.0835
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TAGTTCCTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 405-479 bp 1.0 90.9
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 395-469 bp 1.0 90.3
rps16-trnQ-UUG_p3 CCACATCGTTTCAAGCGAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 375-449 bp 1.0 89.5
rps16-trnQ-UUG_p4 ACCACATCGTTTCAAGCGAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.4 58.1 376-450 bp 1.0 88.1
rps16-trnQ-UUG_p5 TACCACATCGTTTCAAGCGA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 377-451 bp 1.0 87.9